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sgrna targeting 1110004e09rik  (Addgene inc)


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    Addgene inc sgrna targeting 1110004e09rik
    Sgrna Targeting 1110004e09rik, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrna+targeting+1110004e09rik/sgrna+targeting+1110004e09rik/pm34879274-249-97-98
    Average 90 stars, based on 1 article reviews
    sgrna targeting 1110004e09rik - by Bioz Stars, 2026-09
    90/100 stars

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    RNA Sequencing:

    Article Title: Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 T cell expansion and anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI BioLegend Cat#422801 LIVE/DEAD Fixable Near-IR Dead Cell Stain Invitrogen Cat#L34976 Biotin anti-mouse Thy1.1 (OX-7) BioLegend Cat#202510, RRID: AB_2201417 PE anti-mouse Thy1.1 (OX-7) BioLegend Cat#202524, RRID: AB_1595524 APC anti-mouse CD8a (53-6.7) BioLegend Cat#100712, RRID: AB_312751 eFluor 450 anti-mouse CD8a (53-6.7) Invitrogen Cat#48-0081-82, RRID: AB_1272198 PE anti-mouse CD8a (53-6.7) BioLegend Cat#100708, RRID: AB_312747 PE-Cy7 anti-mouse CD8a (53-6.7) Invitrogen Cat#25-0081-82, RRID: AB_469584 PE anti-mouse CD45.2 (104) Invitrogen Cat#12-0454-83, RRID: AB_465679 Brilliant Violet 785 anti-mouse CD45.1 (A20) BioLegend Cat#110743, RRID: AB_2563379 PE anti-mouse ICOS (C398.4A) BioLegend Cat#313508, RRID: AB_416332 FITC anti-mouse CD25 (PC61) BioLegend Cat#102006, RRID: AB_312855 FITC anti-mouse PD-1 (29F.1A12) BioLegend Cat#135214, RRID: AB_10680238 PE anti-mouse PD-1 (29F.1A12) BioLegend Cat#135206, RRID: AB_1877231 PE-Cy7 anti-mouse TIM-3 (RMT3-23) Invitrogen Cat#25-5870-82, RRID: AB_2573483 PerCP-eFluor 710 anti-mouse LAG-3 (C9B7W) Invitrogen Cat#46-2231-82, RRID: AB_11151334 PE-Cy7 anti-mouse CD62L (MEL-14) Invitrogen Cat#25-0621-82, RRID: AB_469633 APC anti-mouse KLRG1 (2F1) BD PharMingen Cat#561620, RRID: AB_10523219 PE anti-mouse CD127 (A7R34) BioLegend Cat#135010, RRID: AB_1937251 FITC anti-mouse IFNg (XMG1.2) BioLegend Cat#505806, RRID: AB_315400 PE anti-mouse TNFa (MP6-XT22) Invitrogen Cat#12-7321-82, RRID: AB_466199 APC anti-mouse GZMB (GB11) Invitrogen Cat#GRB05, RRID: AB_2536539 FITC anti-mouse Ki-67 (SolA15) Invitrogen Cat#11-5698-82, RRID: AB_11151330 PE anti-mouse IRF4 (IRF4.3E4) BioLegend Cat#646403, RRID: AB_2563004 PE Streptavidin BioLegend Cat#405204 APC Streptavidin BioLegend Cat#405243 FITC Streptavidin Invitrogen Cat#11-4317-87 Chemicals, peptides, and recombinant proteins OVA257-264 peptide (SIINFEKL) Chinapeptides Cat#138831-86-4 Actinomycin D CST Cat#15021 Recombinant human IL-2 PeproTech Cat#200-02-1000 Ionomycin Biovision Cat#1566 GolgiStop BD Cat#554724, RRID: AB_2869012 Polybrene Sigma-Aldrich Cat#H9268 Critical commercial assays NEBuilder HiFi DNA Assembly Master Mix NEB Cat#E2621S Q5 High-Fidelity DNA Polymerase NEB Cat#M0491L TIANamp Genomic DNA Kit TIANGEN Cat#DP304 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat#DP430 TIANScript II RT Kit TIANGEN Cat#KR107 Talent qPCR PreMix (SYBR Green) TIANGEN Cat#FP209 Transcription Factor Staining Buffer kit BD PharMingen Cat#562574, RRID: AB_2869424 (Continued on next page) e1 Cell Reports 37, 110083, December 7, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Data files for RNA-seq This paper GEO: GSE186284 Experimental models: Cell lines Phoenix-ECO ATCC Cat#CRL-3214, RRID: CVCL_H717 EL4 ATCC Cat#TIB-39, RRID: CVCL_0255 Experimental models: Organisms/strains C57BL/6 The Jackson Laboratory Cat#JAX:000664, RRID: IMSR_JAX:000664 OT-I transgenic mice (C57BL/6) The Jackson Laboratory Cat#JAX:003831, RRID: IMSR_JAX:003831 Rosa26-Cas9 knockin mice (C57BL/6) The Jackson Laboratory Cat#JAX:026430, RRID: IMSR_JAX:026430 CD45.1 (C57BL/6) The Jackson Laboratory Cat#JAX:002014, RRID: IMSR_JAX:002014 Oligonucleotides sgNon-targeting (sgControl), TTCGCACG ATTGCACCTTGG Doench et al., 2016 Addgene #73632 sgRNA targeting Pdcd1, GACACACGGC GCAATGACAG Doench et al., 2016 Addgene #73632 sgRNA targeting 1110004E09Rik, TGGCC CGCGTCTACAACGGG Doench et al., 2016 Addgene #73632 sgRNA targeting Gm10406, CTGGCTATA AGTGTCCTGTG Doench et al., 2016 Addgene #73632 sgRNA targeting Ndufa2, TCTGATCCGC GAATGCTCGG Doench et al., 2016 Addgene #73632 sgRNA targeting Atp5b, CCCACCCTAG CCACCGACAT Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1, GGATATAGCT GAGAACCTCG Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1(guide#2), ACGT ACGGTAGTGCATGGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Icos, AAATGAAAACAT CCTATGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Irf4, CAAGCAGGACTA CAATCGTG Doench et al., 2016 Addgene #73632 Next-generation sequencing (NGS) primer NGS-F1: AATGATACGGCGACCACCGAG ATCTACACTCTTTCCCTACACGACGCTC TTCCGATCTGCTTTATATATCTTGTGGAA AGGACGAAACACC This paper N/A Next-generation sequencing (NGS) primer NGS-R1: CAAGCAGAAGACGGCATACG AGATAACGTGATGTGACTGGAGTTCAG ACGTGTGCTCTTCCGATCTCCGACTCG GTGCCACTTTTTCAA This paper N/A Recombinant DNA Mouse CRISPR Knockout Pooled Library (Brie) Doench et al., 2016 Addgene #73632 pCL-Eco Addgene Addgene #12371 pMSCV-sgNon-targeting-Thy1.1 (pMSCV-sgControl-Thy1.1) This paper N/A pMSCV-sgPdcd1-Thy1.1 This paper N/A pMSCV-sg1110004E09Rik-Thy1.1 This paper N/A pMSCV-sgGm10406-Thy1.1 This paper N/A pMSCV-sgNdufa2-Thy1.1 This paper N/A (Continued on next page) Cell Reports 37, 110083, December 7, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pMSCV-sgAtp5b-Thy1.1 This paper N/A pMSCV-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIcos-Thy1.1 This paper N/A pMSCV-sgIrf4-Thy1.1 This paper N/A pMSCV-sgIcos-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIrf4-sgRc3h1-Thy1.1 This paper N/A pMIG-IRES-GFP Addgene Addgene #12282 pMIG-Icos-IRES-GFP This paper N/A pMIG-IRES-Thy1.1 This paper N/A pMIG-Irf4-IRES-Thy1.1 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com FlowJo BD https://www.flowjo.com ENCoRE Tr€umbach et al., 2017 https://www.helmholtz-muenchen.de/idg/ research/genetics/bioinformatics/encore/ index.html GSEA https://www.gsea-msigdb.org/gsea/ index.jsp

    Transgenic Assay:

    Article Title: Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 T cell expansion and anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI BioLegend Cat#422801 LIVE/DEAD Fixable Near-IR Dead Cell Stain Invitrogen Cat#L34976 Biotin anti-mouse Thy1.1 (OX-7) BioLegend Cat#202510, RRID: AB_2201417 PE anti-mouse Thy1.1 (OX-7) BioLegend Cat#202524, RRID: AB_1595524 APC anti-mouse CD8a (53-6.7) BioLegend Cat#100712, RRID: AB_312751 eFluor 450 anti-mouse CD8a (53-6.7) Invitrogen Cat#48-0081-82, RRID: AB_1272198 PE anti-mouse CD8a (53-6.7) BioLegend Cat#100708, RRID: AB_312747 PE-Cy7 anti-mouse CD8a (53-6.7) Invitrogen Cat#25-0081-82, RRID: AB_469584 PE anti-mouse CD45.2 (104) Invitrogen Cat#12-0454-83, RRID: AB_465679 Brilliant Violet 785 anti-mouse CD45.1 (A20) BioLegend Cat#110743, RRID: AB_2563379 PE anti-mouse ICOS (C398.4A) BioLegend Cat#313508, RRID: AB_416332 FITC anti-mouse CD25 (PC61) BioLegend Cat#102006, RRID: AB_312855 FITC anti-mouse PD-1 (29F.1A12) BioLegend Cat#135214, RRID: AB_10680238 PE anti-mouse PD-1 (29F.1A12) BioLegend Cat#135206, RRID: AB_1877231 PE-Cy7 anti-mouse TIM-3 (RMT3-23) Invitrogen Cat#25-5870-82, RRID: AB_2573483 PerCP-eFluor 710 anti-mouse LAG-3 (C9B7W) Invitrogen Cat#46-2231-82, RRID: AB_11151334 PE-Cy7 anti-mouse CD62L (MEL-14) Invitrogen Cat#25-0621-82, RRID: AB_469633 APC anti-mouse KLRG1 (2F1) BD PharMingen Cat#561620, RRID: AB_10523219 PE anti-mouse CD127 (A7R34) BioLegend Cat#135010, RRID: AB_1937251 FITC anti-mouse IFNg (XMG1.2) BioLegend Cat#505806, RRID: AB_315400 PE anti-mouse TNFa (MP6-XT22) Invitrogen Cat#12-7321-82, RRID: AB_466199 APC anti-mouse GZMB (GB11) Invitrogen Cat#GRB05, RRID: AB_2536539 FITC anti-mouse Ki-67 (SolA15) Invitrogen Cat#11-5698-82, RRID: AB_11151330 PE anti-mouse IRF4 (IRF4.3E4) BioLegend Cat#646403, RRID: AB_2563004 PE Streptavidin BioLegend Cat#405204 APC Streptavidin BioLegend Cat#405243 FITC Streptavidin Invitrogen Cat#11-4317-87 Chemicals, peptides, and recombinant proteins OVA257-264 peptide (SIINFEKL) Chinapeptides Cat#138831-86-4 Actinomycin D CST Cat#15021 Recombinant human IL-2 PeproTech Cat#200-02-1000 Ionomycin Biovision Cat#1566 GolgiStop BD Cat#554724, RRID: AB_2869012 Polybrene Sigma-Aldrich Cat#H9268 Critical commercial assays NEBuilder HiFi DNA Assembly Master Mix NEB Cat#E2621S Q5 High-Fidelity DNA Polymerase NEB Cat#M0491L TIANamp Genomic DNA Kit TIANGEN Cat#DP304 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat#DP430 TIANScript II RT Kit TIANGEN Cat#KR107 Talent qPCR PreMix (SYBR Green) TIANGEN Cat#FP209 Transcription Factor Staining Buffer kit BD PharMingen Cat#562574, RRID: AB_2869424 (Continued on next page) e1 Cell Reports 37, 110083, December 7, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Data files for RNA-seq This paper GEO: GSE186284 Experimental models: Cell lines Phoenix-ECO ATCC Cat#CRL-3214, RRID: CVCL_H717 EL4 ATCC Cat#TIB-39, RRID: CVCL_0255 Experimental models: Organisms/strains C57BL/6 The Jackson Laboratory Cat#JAX:000664, RRID: IMSR_JAX:000664 OT-I transgenic mice (C57BL/6) The Jackson Laboratory Cat#JAX:003831, RRID: IMSR_JAX:003831 Rosa26-Cas9 knockin mice (C57BL/6) The Jackson Laboratory Cat#JAX:026430, RRID: IMSR_JAX:026430 CD45.1 (C57BL/6) The Jackson Laboratory Cat#JAX:002014, RRID: IMSR_JAX:002014 Oligonucleotides sgNon-targeting (sgControl), TTCGCACG ATTGCACCTTGG Doench et al., 2016 Addgene #73632 sgRNA targeting Pdcd1, GACACACGGC GCAATGACAG Doench et al., 2016 Addgene #73632 sgRNA targeting 1110004E09Rik, TGGCC CGCGTCTACAACGGG Doench et al., 2016 Addgene #73632 sgRNA targeting Gm10406, CTGGCTATA AGTGTCCTGTG Doench et al., 2016 Addgene #73632 sgRNA targeting Ndufa2, TCTGATCCGC GAATGCTCGG Doench et al., 2016 Addgene #73632 sgRNA targeting Atp5b, CCCACCCTAG CCACCGACAT Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1, GGATATAGCT GAGAACCTCG Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1(guide#2), ACGT ACGGTAGTGCATGGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Icos, AAATGAAAACAT CCTATGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Irf4, CAAGCAGGACTA CAATCGTG Doench et al., 2016 Addgene #73632 Next-generation sequencing (NGS) primer NGS-F1: AATGATACGGCGACCACCGAG ATCTACACTCTTTCCCTACACGACGCTC TTCCGATCTGCTTTATATATCTTGTGGAA AGGACGAAACACC This paper N/A Next-generation sequencing (NGS) primer NGS-R1: CAAGCAGAAGACGGCATACG AGATAACGTGATGTGACTGGAGTTCAG ACGTGTGCTCTTCCGATCTCCGACTCG GTGCCACTTTTTCAA This paper N/A Recombinant DNA Mouse CRISPR Knockout Pooled Library (Brie) Doench et al., 2016 Addgene #73632 pCL-Eco Addgene Addgene #12371 pMSCV-sgNon-targeting-Thy1.1 (pMSCV-sgControl-Thy1.1) This paper N/A pMSCV-sgPdcd1-Thy1.1 This paper N/A pMSCV-sg1110004E09Rik-Thy1.1 This paper N/A pMSCV-sgGm10406-Thy1.1 This paper N/A pMSCV-sgNdufa2-Thy1.1 This paper N/A (Continued on next page) Cell Reports 37, 110083, December 7, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pMSCV-sgAtp5b-Thy1.1 This paper N/A pMSCV-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIcos-Thy1.1 This paper N/A pMSCV-sgIrf4-Thy1.1 This paper N/A pMSCV-sgIcos-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIrf4-sgRc3h1-Thy1.1 This paper N/A pMIG-IRES-GFP Addgene Addgene #12282 pMIG-Icos-IRES-GFP This paper N/A pMIG-IRES-Thy1.1 This paper N/A pMIG-Irf4-IRES-Thy1.1 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com FlowJo BD https://www.flowjo.com ENCoRE Tr€umbach et al., 2017 https://www.helmholtz-muenchen.de/idg/ research/genetics/bioinformatics/encore/ index.html GSEA https://www.gsea-msigdb.org/gsea/ index.jsp

    Knock-In:

    Article Title: Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 T cell expansion and anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI BioLegend Cat#422801 LIVE/DEAD Fixable Near-IR Dead Cell Stain Invitrogen Cat#L34976 Biotin anti-mouse Thy1.1 (OX-7) BioLegend Cat#202510, RRID: AB_2201417 PE anti-mouse Thy1.1 (OX-7) BioLegend Cat#202524, RRID: AB_1595524 APC anti-mouse CD8a (53-6.7) BioLegend Cat#100712, RRID: AB_312751 eFluor 450 anti-mouse CD8a (53-6.7) Invitrogen Cat#48-0081-82, RRID: AB_1272198 PE anti-mouse CD8a (53-6.7) BioLegend Cat#100708, RRID: AB_312747 PE-Cy7 anti-mouse CD8a (53-6.7) Invitrogen Cat#25-0081-82, RRID: AB_469584 PE anti-mouse CD45.2 (104) Invitrogen Cat#12-0454-83, RRID: AB_465679 Brilliant Violet 785 anti-mouse CD45.1 (A20) BioLegend Cat#110743, RRID: AB_2563379 PE anti-mouse ICOS (C398.4A) BioLegend Cat#313508, RRID: AB_416332 FITC anti-mouse CD25 (PC61) BioLegend Cat#102006, RRID: AB_312855 FITC anti-mouse PD-1 (29F.1A12) BioLegend Cat#135214, RRID: AB_10680238 PE anti-mouse PD-1 (29F.1A12) BioLegend Cat#135206, RRID: AB_1877231 PE-Cy7 anti-mouse TIM-3 (RMT3-23) Invitrogen Cat#25-5870-82, RRID: AB_2573483 PerCP-eFluor 710 anti-mouse LAG-3 (C9B7W) Invitrogen Cat#46-2231-82, RRID: AB_11151334 PE-Cy7 anti-mouse CD62L (MEL-14) Invitrogen Cat#25-0621-82, RRID: AB_469633 APC anti-mouse KLRG1 (2F1) BD PharMingen Cat#561620, RRID: AB_10523219 PE anti-mouse CD127 (A7R34) BioLegend Cat#135010, RRID: AB_1937251 FITC anti-mouse IFNg (XMG1.2) BioLegend Cat#505806, RRID: AB_315400 PE anti-mouse TNFa (MP6-XT22) Invitrogen Cat#12-7321-82, RRID: AB_466199 APC anti-mouse GZMB (GB11) Invitrogen Cat#GRB05, RRID: AB_2536539 FITC anti-mouse Ki-67 (SolA15) Invitrogen Cat#11-5698-82, RRID: AB_11151330 PE anti-mouse IRF4 (IRF4.3E4) BioLegend Cat#646403, RRID: AB_2563004 PE Streptavidin BioLegend Cat#405204 APC Streptavidin BioLegend Cat#405243 FITC Streptavidin Invitrogen Cat#11-4317-87 Chemicals, peptides, and recombinant proteins OVA257-264 peptide (SIINFEKL) Chinapeptides Cat#138831-86-4 Actinomycin D CST Cat#15021 Recombinant human IL-2 PeproTech Cat#200-02-1000 Ionomycin Biovision Cat#1566 GolgiStop BD Cat#554724, RRID: AB_2869012 Polybrene Sigma-Aldrich Cat#H9268 Critical commercial assays NEBuilder HiFi DNA Assembly Master Mix NEB Cat#E2621S Q5 High-Fidelity DNA Polymerase NEB Cat#M0491L TIANamp Genomic DNA Kit TIANGEN Cat#DP304 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat#DP430 TIANScript II RT Kit TIANGEN Cat#KR107 Talent qPCR PreMix (SYBR Green) TIANGEN Cat#FP209 Transcription Factor Staining Buffer kit BD PharMingen Cat#562574, RRID: AB_2869424 (Continued on next page) e1 Cell Reports 37, 110083, December 7, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Data files for RNA-seq This paper GEO: GSE186284 Experimental models: Cell lines Phoenix-ECO ATCC Cat#CRL-3214, RRID: CVCL_H717 EL4 ATCC Cat#TIB-39, RRID: CVCL_0255 Experimental models: Organisms/strains C57BL/6 The Jackson Laboratory Cat#JAX:000664, RRID: IMSR_JAX:000664 OT-I transgenic mice (C57BL/6) The Jackson Laboratory Cat#JAX:003831, RRID: IMSR_JAX:003831 Rosa26-Cas9 knockin mice (C57BL/6) The Jackson Laboratory Cat#JAX:026430, RRID: IMSR_JAX:026430 CD45.1 (C57BL/6) The Jackson Laboratory Cat#JAX:002014, RRID: IMSR_JAX:002014 Oligonucleotides sgNon-targeting (sgControl), TTCGCACG ATTGCACCTTGG Doench et al., 2016 Addgene #73632 sgRNA targeting Pdcd1, GACACACGGC GCAATGACAG Doench et al., 2016 Addgene #73632 sgRNA targeting 1110004E09Rik, TGGCC CGCGTCTACAACGGG Doench et al., 2016 Addgene #73632 sgRNA targeting Gm10406, CTGGCTATA AGTGTCCTGTG Doench et al., 2016 Addgene #73632 sgRNA targeting Ndufa2, TCTGATCCGC GAATGCTCGG Doench et al., 2016 Addgene #73632 sgRNA targeting Atp5b, CCCACCCTAG CCACCGACAT Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1, GGATATAGCT GAGAACCTCG Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1(guide#2), ACGT ACGGTAGTGCATGGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Icos, AAATGAAAACAT CCTATGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Irf4, CAAGCAGGACTA CAATCGTG Doench et al., 2016 Addgene #73632 Next-generation sequencing (NGS) primer NGS-F1: AATGATACGGCGACCACCGAG ATCTACACTCTTTCCCTACACGACGCTC TTCCGATCTGCTTTATATATCTTGTGGAA AGGACGAAACACC This paper N/A Next-generation sequencing (NGS) primer NGS-R1: CAAGCAGAAGACGGCATACG AGATAACGTGATGTGACTGGAGTTCAG ACGTGTGCTCTTCCGATCTCCGACTCG GTGCCACTTTTTCAA This paper N/A Recombinant DNA Mouse CRISPR Knockout Pooled Library (Brie) Doench et al., 2016 Addgene #73632 pCL-Eco Addgene Addgene #12371 pMSCV-sgNon-targeting-Thy1.1 (pMSCV-sgControl-Thy1.1) This paper N/A pMSCV-sgPdcd1-Thy1.1 This paper N/A pMSCV-sg1110004E09Rik-Thy1.1 This paper N/A pMSCV-sgGm10406-Thy1.1 This paper N/A pMSCV-sgNdufa2-Thy1.1 This paper N/A (Continued on next page) Cell Reports 37, 110083, December 7, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pMSCV-sgAtp5b-Thy1.1 This paper N/A pMSCV-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIcos-Thy1.1 This paper N/A pMSCV-sgIrf4-Thy1.1 This paper N/A pMSCV-sgIcos-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIrf4-sgRc3h1-Thy1.1 This paper N/A pMIG-IRES-GFP Addgene Addgene #12282 pMIG-Icos-IRES-GFP This paper N/A pMIG-IRES-Thy1.1 This paper N/A pMIG-Irf4-IRES-Thy1.1 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com FlowJo BD https://www.flowjo.com ENCoRE Tr€umbach et al., 2017 https://www.helmholtz-muenchen.de/idg/ research/genetics/bioinformatics/encore/ index.html GSEA https://www.gsea-msigdb.org/gsea/ index.jsp

    Next-Generation Sequencing:

    Article Title: Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 T cell expansion and anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI BioLegend Cat#422801 LIVE/DEAD Fixable Near-IR Dead Cell Stain Invitrogen Cat#L34976 Biotin anti-mouse Thy1.1 (OX-7) BioLegend Cat#202510, RRID: AB_2201417 PE anti-mouse Thy1.1 (OX-7) BioLegend Cat#202524, RRID: AB_1595524 APC anti-mouse CD8a (53-6.7) BioLegend Cat#100712, RRID: AB_312751 eFluor 450 anti-mouse CD8a (53-6.7) Invitrogen Cat#48-0081-82, RRID: AB_1272198 PE anti-mouse CD8a (53-6.7) BioLegend Cat#100708, RRID: AB_312747 PE-Cy7 anti-mouse CD8a (53-6.7) Invitrogen Cat#25-0081-82, RRID: AB_469584 PE anti-mouse CD45.2 (104) Invitrogen Cat#12-0454-83, RRID: AB_465679 Brilliant Violet 785 anti-mouse CD45.1 (A20) BioLegend Cat#110743, RRID: AB_2563379 PE anti-mouse ICOS (C398.4A) BioLegend Cat#313508, RRID: AB_416332 FITC anti-mouse CD25 (PC61) BioLegend Cat#102006, RRID: AB_312855 FITC anti-mouse PD-1 (29F.1A12) BioLegend Cat#135214, RRID: AB_10680238 PE anti-mouse PD-1 (29F.1A12) BioLegend Cat#135206, RRID: AB_1877231 PE-Cy7 anti-mouse TIM-3 (RMT3-23) Invitrogen Cat#25-5870-82, RRID: AB_2573483 PerCP-eFluor 710 anti-mouse LAG-3 (C9B7W) Invitrogen Cat#46-2231-82, RRID: AB_11151334 PE-Cy7 anti-mouse CD62L (MEL-14) Invitrogen Cat#25-0621-82, RRID: AB_469633 APC anti-mouse KLRG1 (2F1) BD PharMingen Cat#561620, RRID: AB_10523219 PE anti-mouse CD127 (A7R34) BioLegend Cat#135010, RRID: AB_1937251 FITC anti-mouse IFNg (XMG1.2) BioLegend Cat#505806, RRID: AB_315400 PE anti-mouse TNFa (MP6-XT22) Invitrogen Cat#12-7321-82, RRID: AB_466199 APC anti-mouse GZMB (GB11) Invitrogen Cat#GRB05, RRID: AB_2536539 FITC anti-mouse Ki-67 (SolA15) Invitrogen Cat#11-5698-82, RRID: AB_11151330 PE anti-mouse IRF4 (IRF4.3E4) BioLegend Cat#646403, RRID: AB_2563004 PE Streptavidin BioLegend Cat#405204 APC Streptavidin BioLegend Cat#405243 FITC Streptavidin Invitrogen Cat#11-4317-87 Chemicals, peptides, and recombinant proteins OVA257-264 peptide (SIINFEKL) Chinapeptides Cat#138831-86-4 Actinomycin D CST Cat#15021 Recombinant human IL-2 PeproTech Cat#200-02-1000 Ionomycin Biovision Cat#1566 GolgiStop BD Cat#554724, RRID: AB_2869012 Polybrene Sigma-Aldrich Cat#H9268 Critical commercial assays NEBuilder HiFi DNA Assembly Master Mix NEB Cat#E2621S Q5 High-Fidelity DNA Polymerase NEB Cat#M0491L TIANamp Genomic DNA Kit TIANGEN Cat#DP304 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat#DP430 TIANScript II RT Kit TIANGEN Cat#KR107 Talent qPCR PreMix (SYBR Green) TIANGEN Cat#FP209 Transcription Factor Staining Buffer kit BD PharMingen Cat#562574, RRID: AB_2869424 (Continued on next page) e1 Cell Reports 37, 110083, December 7, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Data files for RNA-seq This paper GEO: GSE186284 Experimental models: Cell lines Phoenix-ECO ATCC Cat#CRL-3214, RRID: CVCL_H717 EL4 ATCC Cat#TIB-39, RRID: CVCL_0255 Experimental models: Organisms/strains C57BL/6 The Jackson Laboratory Cat#JAX:000664, RRID: IMSR_JAX:000664 OT-I transgenic mice (C57BL/6) The Jackson Laboratory Cat#JAX:003831, RRID: IMSR_JAX:003831 Rosa26-Cas9 knockin mice (C57BL/6) The Jackson Laboratory Cat#JAX:026430, RRID: IMSR_JAX:026430 CD45.1 (C57BL/6) The Jackson Laboratory Cat#JAX:002014, RRID: IMSR_JAX:002014 Oligonucleotides sgNon-targeting (sgControl), TTCGCACG ATTGCACCTTGG Doench et al., 2016 Addgene #73632 sgRNA targeting Pdcd1, GACACACGGC GCAATGACAG Doench et al., 2016 Addgene #73632 sgRNA targeting 1110004E09Rik, TGGCC CGCGTCTACAACGGG Doench et al., 2016 Addgene #73632 sgRNA targeting Gm10406, CTGGCTATA AGTGTCCTGTG Doench et al., 2016 Addgene #73632 sgRNA targeting Ndufa2, TCTGATCCGC GAATGCTCGG Doench et al., 2016 Addgene #73632 sgRNA targeting Atp5b, CCCACCCTAG CCACCGACAT Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1, GGATATAGCT GAGAACCTCG Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1(guide#2), ACGT ACGGTAGTGCATGGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Icos, AAATGAAAACAT CCTATGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Irf4, CAAGCAGGACTA CAATCGTG Doench et al., 2016 Addgene #73632 Next-generation sequencing (NGS) primer NGS-F1: AATGATACGGCGACCACCGAG ATCTACACTCTTTCCCTACACGACGCTC TTCCGATCTGCTTTATATATCTTGTGGAA AGGACGAAACACC This paper N/A Next-generation sequencing (NGS) primer NGS-R1: CAAGCAGAAGACGGCATACG AGATAACGTGATGTGACTGGAGTTCAG ACGTGTGCTCTTCCGATCTCCGACTCG GTGCCACTTTTTCAA This paper N/A Recombinant DNA Mouse CRISPR Knockout Pooled Library (Brie) Doench et al., 2016 Addgene #73632 pCL-Eco Addgene Addgene #12371 pMSCV-sgNon-targeting-Thy1.1 (pMSCV-sgControl-Thy1.1) This paper N/A pMSCV-sgPdcd1-Thy1.1 This paper N/A pMSCV-sg1110004E09Rik-Thy1.1 This paper N/A pMSCV-sgGm10406-Thy1.1 This paper N/A pMSCV-sgNdufa2-Thy1.1 This paper N/A (Continued on next page) Cell Reports 37, 110083, December 7, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pMSCV-sgAtp5b-Thy1.1 This paper N/A pMSCV-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIcos-Thy1.1 This paper N/A pMSCV-sgIrf4-Thy1.1 This paper N/A pMSCV-sgIcos-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIrf4-sgRc3h1-Thy1.1 This paper N/A pMIG-IRES-GFP Addgene Addgene #12282 pMIG-Icos-IRES-GFP This paper N/A pMIG-IRES-Thy1.1 This paper N/A pMIG-Irf4-IRES-Thy1.1 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com FlowJo BD https://www.flowjo.com ENCoRE Tr€umbach et al., 2017 https://www.helmholtz-muenchen.de/idg/ research/genetics/bioinformatics/encore/ index.html GSEA https://www.gsea-msigdb.org/gsea/ index.jsp

    Recombinant:

    Article Title: Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 T cell expansion and anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI BioLegend Cat#422801 LIVE/DEAD Fixable Near-IR Dead Cell Stain Invitrogen Cat#L34976 Biotin anti-mouse Thy1.1 (OX-7) BioLegend Cat#202510, RRID: AB_2201417 PE anti-mouse Thy1.1 (OX-7) BioLegend Cat#202524, RRID: AB_1595524 APC anti-mouse CD8a (53-6.7) BioLegend Cat#100712, RRID: AB_312751 eFluor 450 anti-mouse CD8a (53-6.7) Invitrogen Cat#48-0081-82, RRID: AB_1272198 PE anti-mouse CD8a (53-6.7) BioLegend Cat#100708, RRID: AB_312747 PE-Cy7 anti-mouse CD8a (53-6.7) Invitrogen Cat#25-0081-82, RRID: AB_469584 PE anti-mouse CD45.2 (104) Invitrogen Cat#12-0454-83, RRID: AB_465679 Brilliant Violet 785 anti-mouse CD45.1 (A20) BioLegend Cat#110743, RRID: AB_2563379 PE anti-mouse ICOS (C398.4A) BioLegend Cat#313508, RRID: AB_416332 FITC anti-mouse CD25 (PC61) BioLegend Cat#102006, RRID: AB_312855 FITC anti-mouse PD-1 (29F.1A12) BioLegend Cat#135214, RRID: AB_10680238 PE anti-mouse PD-1 (29F.1A12) BioLegend Cat#135206, RRID: AB_1877231 PE-Cy7 anti-mouse TIM-3 (RMT3-23) Invitrogen Cat#25-5870-82, RRID: AB_2573483 PerCP-eFluor 710 anti-mouse LAG-3 (C9B7W) Invitrogen Cat#46-2231-82, RRID: AB_11151334 PE-Cy7 anti-mouse CD62L (MEL-14) Invitrogen Cat#25-0621-82, RRID: AB_469633 APC anti-mouse KLRG1 (2F1) BD PharMingen Cat#561620, RRID: AB_10523219 PE anti-mouse CD127 (A7R34) BioLegend Cat#135010, RRID: AB_1937251 FITC anti-mouse IFNg (XMG1.2) BioLegend Cat#505806, RRID: AB_315400 PE anti-mouse TNFa (MP6-XT22) Invitrogen Cat#12-7321-82, RRID: AB_466199 APC anti-mouse GZMB (GB11) Invitrogen Cat#GRB05, RRID: AB_2536539 FITC anti-mouse Ki-67 (SolA15) Invitrogen Cat#11-5698-82, RRID: AB_11151330 PE anti-mouse IRF4 (IRF4.3E4) BioLegend Cat#646403, RRID: AB_2563004 PE Streptavidin BioLegend Cat#405204 APC Streptavidin BioLegend Cat#405243 FITC Streptavidin Invitrogen Cat#11-4317-87 Chemicals, peptides, and recombinant proteins OVA257-264 peptide (SIINFEKL) Chinapeptides Cat#138831-86-4 Actinomycin D CST Cat#15021 Recombinant human IL-2 PeproTech Cat#200-02-1000 Ionomycin Biovision Cat#1566 GolgiStop BD Cat#554724, RRID: AB_2869012 Polybrene Sigma-Aldrich Cat#H9268 Critical commercial assays NEBuilder HiFi DNA Assembly Master Mix NEB Cat#E2621S Q5 High-Fidelity DNA Polymerase NEB Cat#M0491L TIANamp Genomic DNA Kit TIANGEN Cat#DP304 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat#DP430 TIANScript II RT Kit TIANGEN Cat#KR107 Talent qPCR PreMix (SYBR Green) TIANGEN Cat#FP209 Transcription Factor Staining Buffer kit BD PharMingen Cat#562574, RRID: AB_2869424 (Continued on next page) e1 Cell Reports 37, 110083, December 7, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Data files for RNA-seq This paper GEO: GSE186284 Experimental models: Cell lines Phoenix-ECO ATCC Cat#CRL-3214, RRID: CVCL_H717 EL4 ATCC Cat#TIB-39, RRID: CVCL_0255 Experimental models: Organisms/strains C57BL/6 The Jackson Laboratory Cat#JAX:000664, RRID: IMSR_JAX:000664 OT-I transgenic mice (C57BL/6) The Jackson Laboratory Cat#JAX:003831, RRID: IMSR_JAX:003831 Rosa26-Cas9 knockin mice (C57BL/6) The Jackson Laboratory Cat#JAX:026430, RRID: IMSR_JAX:026430 CD45.1 (C57BL/6) The Jackson Laboratory Cat#JAX:002014, RRID: IMSR_JAX:002014 Oligonucleotides sgNon-targeting (sgControl), TTCGCACG ATTGCACCTTGG Doench et al., 2016 Addgene #73632 sgRNA targeting Pdcd1, GACACACGGC GCAATGACAG Doench et al., 2016 Addgene #73632 sgRNA targeting 1110004E09Rik, TGGCC CGCGTCTACAACGGG Doench et al., 2016 Addgene #73632 sgRNA targeting Gm10406, CTGGCTATA AGTGTCCTGTG Doench et al., 2016 Addgene #73632 sgRNA targeting Ndufa2, TCTGATCCGC GAATGCTCGG Doench et al., 2016 Addgene #73632 sgRNA targeting Atp5b, CCCACCCTAG CCACCGACAT Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1, GGATATAGCT GAGAACCTCG Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1(guide#2), ACGT ACGGTAGTGCATGGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Icos, AAATGAAAACAT CCTATGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Irf4, CAAGCAGGACTA CAATCGTG Doench et al., 2016 Addgene #73632 Next-generation sequencing (NGS) primer NGS-F1: AATGATACGGCGACCACCGAG ATCTACACTCTTTCCCTACACGACGCTC TTCCGATCTGCTTTATATATCTTGTGGAA AGGACGAAACACC This paper N/A Next-generation sequencing (NGS) primer NGS-R1: CAAGCAGAAGACGGCATACG AGATAACGTGATGTGACTGGAGTTCAG ACGTGTGCTCTTCCGATCTCCGACTCG GTGCCACTTTTTCAA This paper N/A Recombinant DNA Mouse CRISPR Knockout Pooled Library (Brie) Doench et al., 2016 Addgene #73632 pCL-Eco Addgene Addgene #12371 pMSCV-sgNon-targeting-Thy1.1 (pMSCV-sgControl-Thy1.1) This paper N/A pMSCV-sgPdcd1-Thy1.1 This paper N/A pMSCV-sg1110004E09Rik-Thy1.1 This paper N/A pMSCV-sgGm10406-Thy1.1 This paper N/A pMSCV-sgNdufa2-Thy1.1 This paper N/A (Continued on next page) Cell Reports 37, 110083, December 7, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pMSCV-sgAtp5b-Thy1.1 This paper N/A pMSCV-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIcos-Thy1.1 This paper N/A pMSCV-sgIrf4-Thy1.1 This paper N/A pMSCV-sgIcos-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIrf4-sgRc3h1-Thy1.1 This paper N/A pMIG-IRES-GFP Addgene Addgene #12282 pMIG-Icos-IRES-GFP This paper N/A pMIG-IRES-Thy1.1 This paper N/A pMIG-Irf4-IRES-Thy1.1 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com FlowJo BD https://www.flowjo.com ENCoRE Tr€umbach et al., 2017 https://www.helmholtz-muenchen.de/idg/ research/genetics/bioinformatics/encore/ index.html GSEA https://www.gsea-msigdb.org/gsea/ index.jsp

    CRISPR:

    Article Title: Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 T cell expansion and anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI BioLegend Cat#422801 LIVE/DEAD Fixable Near-IR Dead Cell Stain Invitrogen Cat#L34976 Biotin anti-mouse Thy1.1 (OX-7) BioLegend Cat#202510, RRID: AB_2201417 PE anti-mouse Thy1.1 (OX-7) BioLegend Cat#202524, RRID: AB_1595524 APC anti-mouse CD8a (53-6.7) BioLegend Cat#100712, RRID: AB_312751 eFluor 450 anti-mouse CD8a (53-6.7) Invitrogen Cat#48-0081-82, RRID: AB_1272198 PE anti-mouse CD8a (53-6.7) BioLegend Cat#100708, RRID: AB_312747 PE-Cy7 anti-mouse CD8a (53-6.7) Invitrogen Cat#25-0081-82, RRID: AB_469584 PE anti-mouse CD45.2 (104) Invitrogen Cat#12-0454-83, RRID: AB_465679 Brilliant Violet 785 anti-mouse CD45.1 (A20) BioLegend Cat#110743, RRID: AB_2563379 PE anti-mouse ICOS (C398.4A) BioLegend Cat#313508, RRID: AB_416332 FITC anti-mouse CD25 (PC61) BioLegend Cat#102006, RRID: AB_312855 FITC anti-mouse PD-1 (29F.1A12) BioLegend Cat#135214, RRID: AB_10680238 PE anti-mouse PD-1 (29F.1A12) BioLegend Cat#135206, RRID: AB_1877231 PE-Cy7 anti-mouse TIM-3 (RMT3-23) Invitrogen Cat#25-5870-82, RRID: AB_2573483 PerCP-eFluor 710 anti-mouse LAG-3 (C9B7W) Invitrogen Cat#46-2231-82, RRID: AB_11151334 PE-Cy7 anti-mouse CD62L (MEL-14) Invitrogen Cat#25-0621-82, RRID: AB_469633 APC anti-mouse KLRG1 (2F1) BD PharMingen Cat#561620, RRID: AB_10523219 PE anti-mouse CD127 (A7R34) BioLegend Cat#135010, RRID: AB_1937251 FITC anti-mouse IFNg (XMG1.2) BioLegend Cat#505806, RRID: AB_315400 PE anti-mouse TNFa (MP6-XT22) Invitrogen Cat#12-7321-82, RRID: AB_466199 APC anti-mouse GZMB (GB11) Invitrogen Cat#GRB05, RRID: AB_2536539 FITC anti-mouse Ki-67 (SolA15) Invitrogen Cat#11-5698-82, RRID: AB_11151330 PE anti-mouse IRF4 (IRF4.3E4) BioLegend Cat#646403, RRID: AB_2563004 PE Streptavidin BioLegend Cat#405204 APC Streptavidin BioLegend Cat#405243 FITC Streptavidin Invitrogen Cat#11-4317-87 Chemicals, peptides, and recombinant proteins OVA257-264 peptide (SIINFEKL) Chinapeptides Cat#138831-86-4 Actinomycin D CST Cat#15021 Recombinant human IL-2 PeproTech Cat#200-02-1000 Ionomycin Biovision Cat#1566 GolgiStop BD Cat#554724, RRID: AB_2869012 Polybrene Sigma-Aldrich Cat#H9268 Critical commercial assays NEBuilder HiFi DNA Assembly Master Mix NEB Cat#E2621S Q5 High-Fidelity DNA Polymerase NEB Cat#M0491L TIANamp Genomic DNA Kit TIANGEN Cat#DP304 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat#DP430 TIANScript II RT Kit TIANGEN Cat#KR107 Talent qPCR PreMix (SYBR Green) TIANGEN Cat#FP209 Transcription Factor Staining Buffer kit BD PharMingen Cat#562574, RRID: AB_2869424 (Continued on next page) e1 Cell Reports 37, 110083, December 7, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Data files for RNA-seq This paper GEO: GSE186284 Experimental models: Cell lines Phoenix-ECO ATCC Cat#CRL-3214, RRID: CVCL_H717 EL4 ATCC Cat#TIB-39, RRID: CVCL_0255 Experimental models: Organisms/strains C57BL/6 The Jackson Laboratory Cat#JAX:000664, RRID: IMSR_JAX:000664 OT-I transgenic mice (C57BL/6) The Jackson Laboratory Cat#JAX:003831, RRID: IMSR_JAX:003831 Rosa26-Cas9 knockin mice (C57BL/6) The Jackson Laboratory Cat#JAX:026430, RRID: IMSR_JAX:026430 CD45.1 (C57BL/6) The Jackson Laboratory Cat#JAX:002014, RRID: IMSR_JAX:002014 Oligonucleotides sgNon-targeting (sgControl), TTCGCACG ATTGCACCTTGG Doench et al., 2016 Addgene #73632 sgRNA targeting Pdcd1, GACACACGGC GCAATGACAG Doench et al., 2016 Addgene #73632 sgRNA targeting 1110004E09Rik, TGGCC CGCGTCTACAACGGG Doench et al., 2016 Addgene #73632 sgRNA targeting Gm10406, CTGGCTATA AGTGTCCTGTG Doench et al., 2016 Addgene #73632 sgRNA targeting Ndufa2, TCTGATCCGC GAATGCTCGG Doench et al., 2016 Addgene #73632 sgRNA targeting Atp5b, CCCACCCTAG CCACCGACAT Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1, GGATATAGCT GAGAACCTCG Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1(guide#2), ACGT ACGGTAGTGCATGGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Icos, AAATGAAAACAT CCTATGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Irf4, CAAGCAGGACTA CAATCGTG Doench et al., 2016 Addgene #73632 Next-generation sequencing (NGS) primer NGS-F1: AATGATACGGCGACCACCGAG ATCTACACTCTTTCCCTACACGACGCTC TTCCGATCTGCTTTATATATCTTGTGGAA AGGACGAAACACC This paper N/A Next-generation sequencing (NGS) primer NGS-R1: CAAGCAGAAGACGGCATACG AGATAACGTGATGTGACTGGAGTTCAG ACGTGTGCTCTTCCGATCTCCGACTCG GTGCCACTTTTTCAA This paper N/A Recombinant DNA Mouse CRISPR Knockout Pooled Library (Brie) Doench et al., 2016 Addgene #73632 pCL-Eco Addgene Addgene #12371 pMSCV-sgNon-targeting-Thy1.1 (pMSCV-sgControl-Thy1.1) This paper N/A pMSCV-sgPdcd1-Thy1.1 This paper N/A pMSCV-sg1110004E09Rik-Thy1.1 This paper N/A pMSCV-sgGm10406-Thy1.1 This paper N/A pMSCV-sgNdufa2-Thy1.1 This paper N/A (Continued on next page) Cell Reports 37, 110083, December 7, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pMSCV-sgAtp5b-Thy1.1 This paper N/A pMSCV-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIcos-Thy1.1 This paper N/A pMSCV-sgIrf4-Thy1.1 This paper N/A pMSCV-sgIcos-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIrf4-sgRc3h1-Thy1.1 This paper N/A pMIG-IRES-GFP Addgene Addgene #12282 pMIG-Icos-IRES-GFP This paper N/A pMIG-IRES-Thy1.1 This paper N/A pMIG-Irf4-IRES-Thy1.1 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com FlowJo BD https://www.flowjo.com ENCoRE Tr€umbach et al., 2017 https://www.helmholtz-muenchen.de/idg/ research/genetics/bioinformatics/encore/ index.html GSEA https://www.gsea-msigdb.org/gsea/ index.jsp

    Knock-Out:

    Article Title: Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 T cell expansion and anti-tumor immunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies DAPI BioLegend Cat#422801 LIVE/DEAD Fixable Near-IR Dead Cell Stain Invitrogen Cat#L34976 Biotin anti-mouse Thy1.1 (OX-7) BioLegend Cat#202510, RRID: AB_2201417 PE anti-mouse Thy1.1 (OX-7) BioLegend Cat#202524, RRID: AB_1595524 APC anti-mouse CD8a (53-6.7) BioLegend Cat#100712, RRID: AB_312751 eFluor 450 anti-mouse CD8a (53-6.7) Invitrogen Cat#48-0081-82, RRID: AB_1272198 PE anti-mouse CD8a (53-6.7) BioLegend Cat#100708, RRID: AB_312747 PE-Cy7 anti-mouse CD8a (53-6.7) Invitrogen Cat#25-0081-82, RRID: AB_469584 PE anti-mouse CD45.2 (104) Invitrogen Cat#12-0454-83, RRID: AB_465679 Brilliant Violet 785 anti-mouse CD45.1 (A20) BioLegend Cat#110743, RRID: AB_2563379 PE anti-mouse ICOS (C398.4A) BioLegend Cat#313508, RRID: AB_416332 FITC anti-mouse CD25 (PC61) BioLegend Cat#102006, RRID: AB_312855 FITC anti-mouse PD-1 (29F.1A12) BioLegend Cat#135214, RRID: AB_10680238 PE anti-mouse PD-1 (29F.1A12) BioLegend Cat#135206, RRID: AB_1877231 PE-Cy7 anti-mouse TIM-3 (RMT3-23) Invitrogen Cat#25-5870-82, RRID: AB_2573483 PerCP-eFluor 710 anti-mouse LAG-3 (C9B7W) Invitrogen Cat#46-2231-82, RRID: AB_11151334 PE-Cy7 anti-mouse CD62L (MEL-14) Invitrogen Cat#25-0621-82, RRID: AB_469633 APC anti-mouse KLRG1 (2F1) BD PharMingen Cat#561620, RRID: AB_10523219 PE anti-mouse CD127 (A7R34) BioLegend Cat#135010, RRID: AB_1937251 FITC anti-mouse IFNg (XMG1.2) BioLegend Cat#505806, RRID: AB_315400 PE anti-mouse TNFa (MP6-XT22) Invitrogen Cat#12-7321-82, RRID: AB_466199 APC anti-mouse GZMB (GB11) Invitrogen Cat#GRB05, RRID: AB_2536539 FITC anti-mouse Ki-67 (SolA15) Invitrogen Cat#11-5698-82, RRID: AB_11151330 PE anti-mouse IRF4 (IRF4.3E4) BioLegend Cat#646403, RRID: AB_2563004 PE Streptavidin BioLegend Cat#405204 APC Streptavidin BioLegend Cat#405243 FITC Streptavidin Invitrogen Cat#11-4317-87 Chemicals, peptides, and recombinant proteins OVA257-264 peptide (SIINFEKL) Chinapeptides Cat#138831-86-4 Actinomycin D CST Cat#15021 Recombinant human IL-2 PeproTech Cat#200-02-1000 Ionomycin Biovision Cat#1566 GolgiStop BD Cat#554724, RRID: AB_2869012 Polybrene Sigma-Aldrich Cat#H9268 Critical commercial assays NEBuilder HiFi DNA Assembly Master Mix NEB Cat#E2621S Q5 High-Fidelity DNA Polymerase NEB Cat#M0491L TIANamp Genomic DNA Kit TIANGEN Cat#DP304 RNAprep Pure Cell/Bacteria Kit TIANGEN Cat#DP430 TIANScript II RT Kit TIANGEN Cat#KR107 Talent qPCR PreMix (SYBR Green) TIANGEN Cat#FP209 Transcription Factor Staining Buffer kit BD PharMingen Cat#562574, RRID: AB_2869424 (Continued on next page) e1 Cell Reports 37, 110083, December 7, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Data files for RNA-seq This paper GEO: GSE186284 Experimental models: Cell lines Phoenix-ECO ATCC Cat#CRL-3214, RRID: CVCL_H717 EL4 ATCC Cat#TIB-39, RRID: CVCL_0255 Experimental models: Organisms/strains C57BL/6 The Jackson Laboratory Cat#JAX:000664, RRID: IMSR_JAX:000664 OT-I transgenic mice (C57BL/6) The Jackson Laboratory Cat#JAX:003831, RRID: IMSR_JAX:003831 Rosa26-Cas9 knockin mice (C57BL/6) The Jackson Laboratory Cat#JAX:026430, RRID: IMSR_JAX:026430 CD45.1 (C57BL/6) The Jackson Laboratory Cat#JAX:002014, RRID: IMSR_JAX:002014 Oligonucleotides sgNon-targeting (sgControl), TTCGCACG ATTGCACCTTGG Doench et al., 2016 Addgene #73632 sgRNA targeting Pdcd1, GACACACGGC GCAATGACAG Doench et al., 2016 Addgene #73632 sgRNA targeting 1110004E09Rik, TGGCC CGCGTCTACAACGGG Doench et al., 2016 Addgene #73632 sgRNA targeting Gm10406, CTGGCTATA AGTGTCCTGTG Doench et al., 2016 Addgene #73632 sgRNA targeting Ndufa2, TCTGATCCGC GAATGCTCGG Doench et al., 2016 Addgene #73632 sgRNA targeting Atp5b, CCCACCCTAG CCACCGACAT Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1, GGATATAGCT GAGAACCTCG Doench et al., 2016 Addgene #73632 sgRNA targeting Rc3h1(guide#2), ACGT ACGGTAGTGCATGGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Icos, AAATGAAAACAT CCTATGAT Doench et al., 2016 Addgene #73632 sgRNA targeting Irf4, CAAGCAGGACTA CAATCGTG Doench et al., 2016 Addgene #73632 Next-generation sequencing (NGS) primer NGS-F1: AATGATACGGCGACCACCGAG ATCTACACTCTTTCCCTACACGACGCTC TTCCGATCTGCTTTATATATCTTGTGGAA AGGACGAAACACC This paper N/A Next-generation sequencing (NGS) primer NGS-R1: CAAGCAGAAGACGGCATACG AGATAACGTGATGTGACTGGAGTTCAG ACGTGTGCTCTTCCGATCTCCGACTCG GTGCCACTTTTTCAA This paper N/A Recombinant DNA Mouse CRISPR Knockout Pooled Library (Brie) Doench et al., 2016 Addgene #73632 pCL-Eco Addgene Addgene #12371 pMSCV-sgNon-targeting-Thy1.1 (pMSCV-sgControl-Thy1.1) This paper N/A pMSCV-sgPdcd1-Thy1.1 This paper N/A pMSCV-sg1110004E09Rik-Thy1.1 This paper N/A pMSCV-sgGm10406-Thy1.1 This paper N/A pMSCV-sgNdufa2-Thy1.1 This paper N/A (Continued on next page) Cell Reports 37, 110083, December 7, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER pMSCV-sgAtp5b-Thy1.1 This paper N/A pMSCV-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIcos-Thy1.1 This paper N/A pMSCV-sgIrf4-Thy1.1 This paper N/A pMSCV-sgIcos-sgRc3h1-Thy1.1 This paper N/A pMSCV-sgIrf4-sgRc3h1-Thy1.1 This paper N/A pMIG-IRES-GFP Addgene Addgene #12282 pMIG-Icos-IRES-GFP This paper N/A pMIG-IRES-Thy1.1 This paper N/A pMIG-Irf4-IRES-Thy1.1 This paper N/A Software and algorithms Prism 8 GraphPad https://www.graphpad.com FlowJo BD https://www.flowjo.com ENCoRE Tr€umbach et al., 2017 https://www.helmholtz-muenchen.de/idg/ research/genetics/bioinformatics/encore/ index.html GSEA https://www.gsea-msigdb.org/gsea/ index.jsp



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